■ 基本信息
別名: | pET21a |
啟動子: | T7 |
復制子: | pBR322 |
終止子: | T7 terminator |
質粒分類: | 大腸桿菌載體；PET系列表達質粒 |
質粒大小: | 5443bp |
質粒標簽: | N-T7, C-His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌BL21(DE3) |
誘導方式: | IPTG或乳糖及其類似物 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pET-21a質粒是一個大腸桿菌表達載體，T7啟動子驅動T7標簽、6×HIS標簽和目的蛋白融合表達。The pET-21a-d(+) vectors carry an N-terminal T7?Tag? sequence plus an optional C-terminal His?Tag? sequence. These vectors differ from pET-24a-d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK151pET-21a大腸表達質粒.txt
